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Hi, I agree that TIFF seems to be a good compromise between saving disk space (by its internal compression) and "processing availability" as input data. Moreover, TIFF is a well established format beyond the cryoEM community and other image processing tools also support it by using the provided library. This is a major downside of more specific solutions proposed. So, from a developer perspective, dealing with TIFF files (compressed or not) will be transparent and there are many more tools for users as well. Best, Jose Miguel On Sun, Jun 3, 2018 at 10:16 PM, Takanori Nakane <[log in to unmask]> wrote: > Hi, > > Yes, for long-term 'cold' storage, (p)bzip2 can be better. > > The advantage of TIFF for processing is that it is being supported > by many programs (MotionCor2, cisTEM, RELION 3, etc) and > compression/decompression is handled transparently. Otherwise, users > have to decompress huge movies before processing. > > In RELION 3, our new motion refinement directly reads raw movie frames > (in MRCS or TIFF). You no longer have to write aligned movies and > movie particles. So if you record images in TIFF from the beginning, > you can save lot of storage. > > Best regards, > > Takanori Nakane > > > On 2018/06/03 14:15, Oliver Clarke wrote: > >> I would vote for bzip2 over compressed tiff. >> >> For a test 50 frame, 1.4G stack, pbzip2 gives a 214M archive, while >> mrc2tif gives a 264M tiff - and pbzip2 is much faster to >> compress/decompress at least on my system (GPU workstation with 24 cores/48 >> threads) >> >> Cheers >> Oli >> >> On Jun 3, 2018, at 9:00 AM, Dimitry Tegunov <[log in to unmask]> wrote: >>> >>> Hey! >>> >>> In addition to what Takanori wrote: If you're stuck with EPU for data >>> collection, this tool can run on the K2 computer to convert >>> gain-uncorrected MRCs to compressed TIFFs on-the-fly: >>> https://github.com/dtegunov/stacker2. It will also happily compress >>> your old files, assuming they contain only integers. >>> >>> I very much hope that one day we will get an EPU version with TIFF >>> support for K2/3 data. And with most of SerialEM's functionality. And a >>> pony. In this exact order, please ;-) >>> >>> Cheers, >>> Dimitry >>> >>> ######################################################################## >>> >>> To unsubscribe from the CCPEM list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 >>> >> >> ######################################################################## >> >> To unsubscribe from the CCPEM list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 >> >> > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1