Dear Nina,
I think the first thing you need to check is how the data looks in the spectrometer. When you look at the spectra in Topspin, do they overlap perfectly?
Best wishes,
Tharin
----------------------------------------
Dr. Tharin Blumenschein, Lecturer
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School of Chemistry
University of East Anglia
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From: "CcpNmr software mailing list <[log in to unmask]>" <[log in to unmask]> on behalf of Nina Christou <[log in to unmask]>
Reply-To: "CcpNmr software mailing list <[log in to unmask]>" <[log in to unmask]>
Date: Thursday, 31 May 2018 13:51
To: "CcpNmr software mailing list <[log in to unmask]>" <[log in to unmask]>
Subject: Pseudo3D spectrum import problem
Dear Sir/Madam,
My name is Nina Christou and I'm currently using CCPN Analysis 2.4.2 to
analyse some Pseudo 3D data I collected of an Hydrogen-Deuterium
exchange experiment I recorded. It seems that the data are partly ruined
when I import them in Bruker format so, as a result, I cannot perform my
analysis.
Namely, I have a series of HN spectra collected (457 to be exact)
overtime. I import them directly from the acquired spectra from a Bruker
spectrometer. I have a similar set of data which I imported last week
and everything worked perfectly. However, this time, it seems like the
spectra going from plane to plane are different. I am attaching some
pictures which show a series of some planes, from the lowest to the
highest, so you can have an idea of what I'm talking about. I have a
reference spectrum (pink) which should overlap with the mauve spectra
(pseudo-3D). Going from the lowest to the highest plane, the intensity
of the peaks should decrease but not shift.
As a result of this weird read out of my data (spectra is shifting and
some peaks are 'halved'), instead of having a normal exponential decay
of the intensity of each peak overtime, I get a graph starting at noise
level intensity, followed by some zeros and eventually an exponential
decay. I have included a graph of such a peak to aid to your understanding.
Can you let me know how I could potentially fix that? I have run out of
ideas myself.
Sincerely,
Nina
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