Hi,
If you send more details on MRC to MTZ problem to
[log in to unmask] then we can have a look.
cheers
Martyn
From: Collaborative Computational Project in Electron cryo-Microscopy
[mailto:[log in to unmask]] On Behalf Of Chiara Rapisarda
Sent: 14 May 2018 14:00
To: [log in to unmask]
Subject: [ccpem] 3D refinement times/ mrc to mtz crash
Dear all,
we are using relion to do a 3D refinement of asymmetric particles with box size 400 with a pixel size of 1.15 Å and the iterations with 30k particles take 30-45
minutes, while with 120k particles they take 17 hours or more.
Is there something that we are doing wrong? It seems like a disproportionate amount of time for just 3 times the number of particles.
Does anybody have any tips to speed things up?
Also when trying to do convert class-001.mrc to mtz on ccpem, the program crashes and I cannot find in the output a reason why. Can anybody help?
Thanks,
Chiara Rapisarda
Post doc
Université de Bordeaux, CNRS, INSERM
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Tel. +33 (5) 4000 3617
Institut européen de chimie et biologie
2, rue Robert Escarpit
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www.iecb.u-bordeaux.fr