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Hi,

You can use
 relion_helix_toolbox --check --boxdim [] --helical_outer_diameter [] --rise_max [] --sphere_percentage (or particle diameter) []

To get the range for z.

Best,
Hamid



Sent via the Samsung Galaxy S8, an AT&T 4G LTE smartphone

-------- Original message --------
From: Yangqi Gu <[log in to unmask]>
Date: 5/10/18 10:10 AM (GMT-05:00)
To: [log in to unmask]
Subject: [ccpem] Helical refinement error

Hi all,
I am using Relion 2.1 to do a helical refinement. I am using following parameters for the helical refinement:
--healpix_order 3 \
                --auto_local_healpix_order 4 \
                --offset_range 5 \
                --offset_step 2 \
                --sym C1 \
                --low_resol_join_halves 40 \
                --norm \
                --scale  \
                --helix \
                --helical_outer_diameter 60 \
                --helical_nr_asu 2 \
                --helical_twist_initial 105 \
                --helical_rise_initial 10 \
                --helical_z_percentage 0.1 \
                --helical_symmetry_search \
                --helical_twist_min 100 \
                --helical_twist_max 120 \
                --helical_twist_inistep 0.02 \
                --helical_rise_min 5 \
                --helical_rise_max 20 \
                --helical_rise_inistep 0.01 \
                --sigma_tilt 5 \
But some how, I had issue with the z percentage 0.1. When I did refinement, it said "Z percentage is out of range under current settings!". I wanted to know which parameters violate the setting rules. Could you help me out?