Hi Zach,
It is hard to judge from one screen shot, maybe a double conformation of
the Asn and the Trp immediately before it?
Regards,
Napo
Em 2017-08-18 18:17, Maben, Zachary escreveu:
> Hello,
>
> I am refining a few datasets of my protein of interest (ERAP1) bound to various inhibitors, and I repeatedly observe a positive density peak near an asparagine. I first thought this might be a glycan, but this site does not fit the canonical N-linked glycosylation motif (this site sequence is NKL). Previous crystal structures of this protein do not identify any ligand at this position.
>
> https://www.dropbox.com/sh/2wdax5uqlb1f4m3/AABQlGK2_mgibtqnm61E-7o0a?dl=0
>
> [1]
>
> Asn833 positive density [1]
> www.dropbox.com
> Shared with Dropbox
>
> I would appreciate any suggestions of what this density might be. Thank you for your help.
>
> Best,
>
> Zach Maben
>
> Stern Lab
>
> Pathology Department
>
> University of Massachusetts Medical School
Links:
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[1]
https://www.dropbox.com/sh/2wdax5uqlb1f4m3/AABQlGK2_mgibtqnm61E-7o0a?dl=0
