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Hello, FSLview can have problems correctly displaying the latter volumes of large NIFTI files. As a double check you can use fslsplit to split the 4D file into its 3D constituents - if the end volume of the “split” images matches the original input, then the 4D should be good to go.. Kind Regards Matthew > On 4 Apr 2017, at 17:50, tom parker <[log in to unmask]> wrote: > > Dear FSL experts, > > I am trying to merge the PET images of 200 old subjects into a 4D file to feed it into randomise and run some statistical analyses. > > However, when i use: > > fslmerge -t output.nii.gz subjects/*.nii.gz > > And then open in fslview the output.nii.gz 4D file, i see that the last subjects are not merged well, although i checked their images and they seem fine. > > Should I use a different command to merge the files? > Or is there a limit to the number of files that can be merged together? > > Thank you so much! >