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Dear Xu, If your interaction is significant, it means that there is a difference somewhere. The F-contrast will not tell you which it is, so to interpret the interaction run additional t-tests for the contrasts below: A1:B1 versus A1:B2 A2:B1 versus A2:B2 A3:B1 versus A3:B2 A1:B1 versus A2:B1 A2:B1 versus A3:B1 A1:B1 versus A3:B1 A1:B2 versus A2:B2 A2:B2 versus A3:B2 A1:B2 versus A3:B2 best, Amber Date: Wed, 9 Sep 2015 10:45:38 +0200 From: [log in to unmask] Subject: [SPM] 2*3 full factorial design To: [log in to unmask] Dear SPM experts, My experiment is a 2*3 within-subject design. In the 2nd-level analysis, I use the 2*3 full factorial model. I found the brain activation in the interaction(F-contrast). However, I am not sure how to explain this 2*3 interaction? Would you please give me more information about this? Thanks! Best regards,Xu