 |
 |
Thanks Donald. I was also told that atrophy changes with age, we have mixed animals with different age group, I probably have to scan more animals with small age group.
Thanks
Ned
From: SPM (Statistical Parametric Mapping) [mailto:[log in to unmask]] On Behalf Of MCLAREN, Donald
Sent: Thursday, June 25, 2015 1:40 PM
To: [log in to unmask]
Subject: Re: [SPM] VBM results
It's hard to know if this is what to expect. The differences are likely to be different for each knockout mouse line depending on the gene being knocked out. If you are concerned about the lack of significance, maybe the controls and knockouts aren't that different OR you might want to try another p-value and cluster size that would still correct for multiple comparisons.
Best Regards, Donald McLaren
=================
D.G. McLaren, Ph.D.
Research Fellow, Department of Neurology, Massachusetts General Hospital and
Harvard Medical School
Postdoctoral Research Fellow, GRECC, Bedford VA
Website: http://www.martinos.org/~mclaren
Office: (773) 406-2464
=====================
This e-mail contains CONFIDENTIAL INFORMATION which may contain PROTECTED
HEALTHCARE INFORMATION and may also be LEGALLY PRIVILEGED and which is
intended only for the use of the individual or entity named above. If the
reader of the e-mail is not the intended recipient or the employee or agent
responsible for delivering it to the intended recipient, you are hereby
notified that you are in possession of confidential and privileged
information. Any unauthorized use, disclosure, copying or the taking of any
action in reliance on the contents of this information is strictly
prohibited and may be unlawful. If you have received this e-mail
unintentionally, please immediately notify the sender via telephone at (773)
406-2464 or email.
On Tue, Jun 23, 2015 at 8:17 AM, Ned <[log in to unmask]> wrote:
Hello SPM VBM experts
I tried to compare the differences between two mouse groups ( control vs knockout), I was able to do registration, normalization and segmentation nicely. I did 0.5 mm isotropic smoothing Then I did 2 sample t test on grey matter with p=0.01 with 200 clusters, I got the above results. I am wondering that why I am getting few clusters with significant voxels? Does this look normal? Please share your suggestions.
Thanks
Ned