If you use realignment in SPM, then you should use these images as the input into normalization (FSL or SPM). I would not repeat the realignment in FSL and then do normalization in FSL. You also need to smooth the data after normalization.I don't see why you would use SPM for some pre-processing, then switch to FSL for a single step, then switch back for smoothing. I would do all the pre-processing in FSL if you prefer the FSL normalization.SPM uses a non-linear normalization approach. The non-linear approach is actually an affine transform+non-linear deformations. This is similar to FNIRT in FSL.As to whether to include the motion parameters in the analysis. The inclusion of the motion parameters usually reduces the noise in the model and could lead to more accurate estimates; however, if you move with each stimulus, then the motion would be correlated with the predicted HRF and this would reduce the accuracy of the estimates. As long as you don't have stimulus correlated motion, I would include them as a covariate.Best Regards, Donald McLaren
D.G. McLaren, Ph.D.
Research Fellow, Department of Neurology, Massachusetts General Hospital and
Harvard Medical School
Postdoctoral Research Fellow, GRECC, Bedford VA
Office: (773) 406-2464
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406-2464 or email.On Mon, Jun 8, 2015 at 6:59 AM, Joelle Zimmermann <[log in to unmask]> wrote:Hi Luis,Thanks very much for your response. So you would suggest:1) doing realignment in SPM on the non-normalized images, to get the rp.txt motion parameter file2) doing normalization in FSL. (question: would this be done on the realigned images from SPM or the original?)3) doing a first-level analysis in SPM on the normalized (from FSL) images, with the rp.txt file that I made in step #1 as an additional regressorIs that right?A few more questions:How necessary/standard procedure is it to include the realignment parameters (the rp.txt) as additional regressors?In the GUI, does SPM do normalization in a linear way (ie linear registration?).Thanks,JoelleOn Mon, Jun 8, 2015 at 12:32 PM, Luis Morís <[log in to unmask]> wrote:Hi Joelle,Why not take the non normalized images and do the realignment on them? Then you can normalize the images after realigning them using FSL.I wouldn't expect the realignment parameters calculated this way to be very accurate. In fact I would expect them to be artificially close as when normalizing you are bringing images into a template space, and therefore realigning them indirectly.Cheers,LuisOn Mon, Jun 8, 2015 at 11:39 AM, Joelle Zimmermann <[log in to unmask]> wrote:Hi - I'm doing a first-level analysis of fMRI data in SPM, and want to use realignment parameters (rp text file in the preprocessing folder) as additional regressors (ie select the rp file as "Multiple regressor").
However, I've already done normalization in FSL (using nonlinear registration).
Can I input the already normalized 4D functional to SPM, and run realignment on this in SPM, to get that rp text file that I can later input as a regressor?
I understand that in SPM, the procedure is usually to do realignment (followed by coregistration, segmentation), and only then normalization.