The xtal is needle cluster and I cam see UV for the centre.2015-02-18 12:15 GMT+00:00 Fang Lu <[log in to unmask]>:Hi all,I got this odd diffraction. Not sure what it is, detergent or protein? Any ideas?The crystallisation condition is 2.4M Sodium Malonate.GF buffer contains HEGA-10,Tris, KAcetate, MgAcetate, EDTA and DTT.Protein size is around 100kDa.Thank you for your time.Fang