 |
 |
That is a fantastically low Rmerge ! What are the actual numbers? You might expectslightly higher Rmerge for stronger reflections? i.e. at low resin.. Eleanor On 27 February 2015 at 20:40, Veronica Pillar <[log in to unmask]> wrote: > Hi all, > > I have a data set from a large room-temperature lysozyme crystal > consisting of 6 90-degree sweeps, each taken from a fresh spot on the > crystal. When I scale & merge the first sweep by itself, the R[meas/merge] > vs. resolution trace as reported by aimless looks fairly normal (lowest > values in the 6-2.5 A range and steadily increasing at higher resolution). > However, as I look at the subsequent sweeps individually, the traces get > progressively stranger until the final sweep's R vs. resolution trace looks > like a smiley face, lowest around 2.5 A and about the same in the lowest > and highest resolution bins. The overall Rmeas is about the same for all > sweeps (3.0-3.2%). Do you have any ideas on what has happened (either > during data collection or data processing) to cause this? > > Thanks, > Veronica >