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Dear Sjors, I've just started learning Relion 1.2 using the tutorial you provided, but the run is crushing after several iteration (both 2d and 3d classification) with the following error: ============================= > Expectation iteration 6 of 25 > 60/ 60 sec > ............................................................~~(,_,"> > Maximization ... > 000/??? sec ~~(,_,"> > [oo] DIRECT_A1D_ELEM(sigma2, i)= -468.511 > BackProjector::reconstruct: ERROR: unexpectedly small, yet non-zero sigma2 > value, this should not happen...a > File: src/backprojector.cpp line: 668 > -------------------------------------------------------------------------- > MPI_ABORT was invoked on rank 2 in communicator MPI_COMM_WORLD > with errorcode 1. I'm not sure if this can be related<http://www2.mrc-lmb.cam.ac.uk/relion/index.php/FAQs#My_runs_keep_crashing_at_seemingly_random_points_in_the_refinement>to the work of OpenMPI on our cluster... Best regards, Grigory Sharov, Institut de Génétique et de Biologie Moléculaire et Cellulaire Departement of Structural Biology and Genomics 1, rue Laurent Fries 67404 Illkirch, France tel. 03 88 65 57 48 e-mail: [log in to unmask]