Position:
Group Leader– Structural Biology – Protein Chemistry
Client: Dart
NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company
with offices in San Diego, CA. DNS will
discover new technologies and develop new therapies to help maintain cognitive
vitality throughout life. www.dartneuroscience.com
DNS is seeking a group leader
with extensive experience in the development and implementation of protein
purification processes for macromolecular crystallography studies.
Reporting to the director of
structural biology, the successful candidate will be responsible to establish
and manage the protein purification team within the group.
The candidate should have
excellent knowledge of the state-of-the-art protein purification equipment and
processes necessary to create and mentor others in establishing purification
procedures that reproducibly lead to the generation of crystallization-grade
protein in quantity and quality suitable for structural studies.
Major responsibilities will
include independent hands-on experimental work and leading a team of scientists
and research associates to support large scale protein purifications of proteins
recombinantly expressed in either prokaryotic or eukaryotic (baculovirus and
mammalian cells) systems and originating from different protein families
including membrane proteins.
The candidate will be
required to provide scientific expert advice to group members and have frequent
interactions with the molecular biologists, protein production facility and
crystallographers in the group.
The candidate will be
required to provide feedback to team members to maximize protein yields and
quality that will lead to successful crystallization enabling 3D structure
determination.
Good communication skills,
leadership skills, project and sample management experience overseeing multiple
projects in parallel as well as capacity for strategic thinking are required.
Requirements & Responsibilities:
·
Ph.D.
in life science with thesis.
·
8+
years of relevant experience in protein purification and biophysical
characterization of proteins originating from different protein families for
crystallization and 3D-structure determination.
·
Preferably
1+ year of industrial supervisory experience of Ph.D. scientists and research
associates.
·
Proven
hands-on experience in different protein purification methods (e.g. affinity, size-exclusion, ion-exchange,
isoelectric focusing chromatography) and biochemical/biophysical
characterization techniques (e.g., Western blot, light scattering, calorimetry,
analytical ultracentrifugation, DLS, thermal melting, protein MS, protein NMR).
·
Expert
knowledge in the scientific principles and concepts of protein chemistry and
good understanding of the protein quality requirements for crystallization
purposes preferably demonstrated through peer-reviewed publications.
·
Knowledge
in membrane protein chemistry and other areas of structural biology (molecular
biology and crystallography) is desirable.
·
Inter-disciplinary
knowledge of drug discovery (enzymology, cellular pharmacology, DMPK) is a
plus.
·
Demonstrated
independent thought/creativity in science.
Excellent collaboration, communication (oral and written) and
multitasking skills and able to present work formally to Scientific Research
Committees.
Opportunity: DNS offers competitive salaries and a great benefits package
including incentive bonuses, healthcare, vision, dental, long-term disability,
life insurance and a 401(k) retirement savings plan.For Additional Information Contact: Bill Domann
