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One more option:

phenix.fetch_pdb 1akg --maps

will fetch structure and reflection data files from PDB and generate
2mFo-DFc and mFo-DFc maps (as well as anomalous difference map if
reflection data is anomalous).

Pavel

On Mon, Feb 4, 2013 at 5:52 AM, Robbie Joosten
<[log in to unmask]>wrote:

> Hi Bhat,
>
> You can run Refmac for 0-cycles to make the maps. If you use unrestrained
> refinement, you don't have to mess with restraint files. There is also an
> option in Coot to do this.
> But if you are in Coot anyway, you might as well get the maps from EDS
> directly or (after installing a plugin:
> http://www.cmbi.ru.nl/pdb_redo/coot.html) get a re-refined model and map
> form PDB_REDO.
>
> Cheers,
> Robbie
>
> > -----Original Message-----
> > From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
> > bhat
> > Sent: Monday, February 04, 2013 14:16
> > To: [log in to unmask]
> > Subject: [ccp4bb] generating electron density from PDB and structure
> factor
> > file
> >
> > Dear All,
> >
> > I  would like to know what is the best possible way to generate the
> density
> > from the published pdb file.
> >
> > thanks,
> >
> > Bhat
>