One more option: phenix.fetch_pdb 1akg --maps will fetch structure and reflection data files from PDB and generate 2mFo-DFc and mFo-DFc maps (as well as anomalous difference map if reflection data is anomalous). Pavel On Mon, Feb 4, 2013 at 5:52 AM, Robbie Joosten <[log in to unmask]>wrote: > Hi Bhat, > > You can run Refmac for 0-cycles to make the maps. If you use unrestrained > refinement, you don't have to mess with restraint files. There is also an > option in Coot to do this. > But if you are in Coot anyway, you might as well get the maps from EDS > directly or (after installing a plugin: > http://www.cmbi.ru.nl/pdb_redo/coot.html) get a re-refined model and map > form PDB_REDO. > > Cheers, > Robbie > > > -----Original Message----- > > From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of > > bhat > > Sent: Monday, February 04, 2013 14:16 > > To: [log in to unmask] > > Subject: [ccp4bb] generating electron density from PDB and structure > factor > > file > > > > Dear All, > > > > I would like to know what is the best possible way to generate the > density > > from the published pdb file. > > > > thanks, > > > > Bhat >