Tim,
My understanding is that the D-isomer does not get incorporated. I could be wrong though. Bacteria may convert the D to the L-form.
Chitta
----- Original Message -----
From: "Tim Gruene" <[log in to unmask]>
To: [log in to unmask]
Sent: Monday, November 12, 2012 4:25:34 AM
Subject: Re: [ccp4bb] seleno methionine labelling of protein
-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1
Dear ,
I am not up to date, but is there an expression system that allows you
to incorporate D-amino acids into proteins? In terms of phasing only
the Se-atom is a point or sphere, so the signal won't be altered
provided the position of that atom is the same in either case.
Best,
Tim
On 11/12/2012 10:06 AM, Faisal Tarique wrote:
> Dear All
>
> just want to ask the difference between labelling your protein with
> D L selenomethionine mixture and L selenomethionine alone..will it
> make difference in anomalous diffraction,detection of Se signal and
> extraction of phases. Or will be the same for both..?
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.12 (GNU/Linux)
Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/
iD8DBQFQoMCOUxlJ7aRr7hoRAopyAKD4kBksPb4zIDYDgok5r5BuuefndgCgrf3S
r+YIxyjC50XywK1Tgc+fglk=
=3GcK
-----END PGP SIGNATURE-----