Tim,


My understanding is that the D-isomer does not get incorporated. I could be wrong though. Bacteria may convert the D to the L-form.

Chitta

----- Original Message -----
From: "Tim Gruene" <[log in to unmask]>
To: [log in to unmask]
Sent: Monday, November 12, 2012 4:25:34 AM
Subject: Re: [ccp4bb] seleno methionine labelling of protein

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Dear     ,

I am not up to date, but is there an expression system that allows you
to incorporate D-amino acids into proteins? In terms of phasing only
the Se-atom is a point or sphere, so the signal won't be altered
provided the position of that atom is the same in either case.

Best,
Tim

On 11/12/2012 10:06 AM, Faisal Tarique wrote:
> Dear All
>
> just want to ask the difference between labelling your protein with
> D L selenomethionine mixture and L selenomethionine alone..will it
> make difference in anomalous diffraction,detection of Se signal and
> extraction of phases. Or will be the same for both..?
>

- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

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