 |
 |
thanx all for their nice suggestions On Mon, Nov 12, 2012 at 6:23 PM, Chittaranjan Das <[log in to unmask]> wrote: > Tim, > > > My understanding is that the D-isomer does not get incorporated. I could > be wrong though. Bacteria may convert the D to the L-form. > > Chitta > > > > ----- Original Message ----- > From: "Tim Gruene" <[log in to unmask]> > To: [log in to unmask] > Sent: Monday, November 12, 2012 4:25:34 AM > Subject: Re: [ccp4bb] seleno methionine labelling of protein > > -----BEGIN PGP SIGNED MESSAGE----- > Hash: SHA1 > > Dear , > > I am not up to date, but is there an expression system that allows you > to incorporate D-amino acids into proteins? In terms of phasing only > the Se-atom is a point or sphere, so the signal won't be altered > provided the position of that atom is the same in either case. > > Best, > Tim > > On 11/12/2012 10:06 AM, Faisal Tarique wrote: > > Dear All > > > > just want to ask the difference between labelling your protein with > > D L selenomethionine mixture and L selenomethionine alone..will it > > make difference in anomalous diffraction,detection of Se signal and > > extraction of phases. Or will be the same for both..? > > > > - -- > - -- > Dr Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A > > -----BEGIN PGP SIGNATURE----- > Version: GnuPG v1.4.12 (GNU/Linux) > Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ > > iD8DBQFQoMCOUxlJ7aRr7hoRAopyAKD4kBksPb4zIDYDgok5r5BuuefndgCgrf3S > r+YIxyjC50XywK1Tgc+fglk= > =3GcK > -----END PGP SIGNATURE----- > -- Regards Faisal School of Life Sciences JNU