Native PAGE (i.e. BN-PAGE), light scattering (i.e. MALLS)

Not quick and easy but could work: AUC (i.e. a sedimentation equilibrium experiment)

-Brad

On Tue, Jun 26, 2012 at 9:01 AM, Careina Edgooms <[log in to unmask]> wrote:

Dear ccp4 bulletin board

I apologise for off topic question. I wonder if anybody knows of a good method to detect oligomerisation? 

I suspect an equilibrium intermediate is forming oligomers based on tryptophan fluorescence showing an exposed tryptophan becoming buried in a hydrophobic region. I would like to perform some experiments to prove this. Cross linking is not working for me and neither is SEC HPLC due to technical issues with the urea I think.

Are there any other quick and easy techniques that any of you can think of off the cuff? Shifts in equilibrium unfolding curves done at different concentrations will probably show something because of the law of mass action but this is a big job needing many replicates so I wonder if there are any other simple methods available?

Thanks

Careina