Hi Jun,How fast do you need the temperature to be changed? One easy way to get very fast temperature change is to prepare water at different temperatures, and circulate the water around your chamber, which of course need to be watertight. If you can find valves that can take computer instructions then you can control the sequence of the water of different temperatures going around your chamber. If you have a constant temperature hot water supply and ice water supply and are not t.o concerned about wasting water, then two proportional valves can do what you want: mix the ice water with the hot water at different ratios then you can get to anywhere in the 0-50C range.I doubt there would be solid block based devices that would allow you to quickly and evenly change your chamber's temperature, as this would need even and efficient heating and cooling of your chamber on all 6 faces, which is difficult to do with solid surfaces.ZhijieFrom: [log in to unmask]" href="mailto:[log in to unmask]" target="_blank">Jun MaSent: Wednesday, June 13, 2012 3:36 PMTo: [log in to unmask]" href="mailto:[log in to unmask]" target="_blank">Zhijie LiSubject: Re: [ccp4bb] Where to get a thermo-cycling controlled chamberHi Zhijie,Thank you for your detailed reply. I'm sorry i didn't describe as clearly as possible. I'm trying to make 2D-crystal, and using a special apparatus, not so big, but cannot fit a regular thermocycler designing for 96-wells plate/PCR. So I need a kind of chamber, in which I can put my apparatus. Do you have any idea about this?Jun2012/6/13 Zhijie Li <[log in to unmask]>
Hi Jun£¬In this following article an metallic adapter was used to mount a 192 well sitting drop plate on a conventional thermocycler.However I guess there would be significant evaporation and condensation if you use vapor diffusion setup and cycle the temperatures.On the other hand, if you do microbatch under oil, then you should be able to easily setup the experiment on a 96-tube PCR plate and mount it on a PCR machine. I guess the temperature can be more precisely controlled this way with no special equipment needed. Especially, the evaporation and condensation during thermo cycles would probably be less problematic.ZhijieFrom: [log in to unmask]" href="mailto:[log in to unmask]" target="_blank">Jun MaSent: Tuesday, June 12, 2012 11:15 PMTo: [log in to unmask]" href="mailto:[log in to unmask]" target="_blank">[log in to unmask]Subject: [ccp4bb] Where to get a thermo-cycling controlled chamberHi everybody,My experiment need me to try a thermo-cycling method for protein crystallization. So I need a kind of temperature chamber, which can changes temperature quickly, precisely and steadily. Furthermore, it should be relatively quiet when running, because I'm afraid vibration will affect the crystallization of my protein. I just need a small one, which can control temperature from 0 C to 50 C.I'm looking for this for some time, and still don't know where to get it. If anyone can provide information about where to purchase, or how to solve the thermo-cycling control if there's no commercial merchandise, I will appreciate that very much.
Any other suggestions are welcome.
Jun Ma2012-06-12