HI, I have been following the thread (Torsten and Donald) about generating second-level difference images for repeated measures analyses and I am still a little unclear as to the correct way to generate these for subsequent input to the third-level Of the three second-level options listed below (first two put forward by Torsten) which is correct? Option 1) EV1(scan) EV2(mean) 1 1 -1 1 contrast 1: 1 0 Option 2) EV1(scan1) EV2(scan2) 1 0 0 1 contrast 1: 1 -1 Option3) EV1(scan) 1 -1 contrast 1: 1 Many thanks