Dear Aditya, The segmentations we produce are partial volume segmentations, so it is not enough to know how many non-zero voxels there are. The values in the voxels represent partial volumes and these should be added up to give the most accurate estimation. We definitely see accuracy advantages by doing things this way. That is why the values need to be multiplied. As for TIV, you can calculate the WM and CSF volumes the same way, but the bounding CSF is highly influenced by the mask used, which comes from BET. So all you get with this method is the volume of the BET mask which is not tuned to give a very precise measurement of TIV. Hence we don't recommend this for very precise measurements. For normalising by TIV this can be reasonable to do, but it does depend on the quality of your BET results. For documentation, the main source is http://www.fmrib.ox.ac.uk/fsl/fslvbm/index.html and what is on the email list. I don't think there is any specific documentation just about the files that are produced. The ones of interest are detailed in the relevant sections of the above documentation. All the best, Mark On 27 May 2011, at 16:50, Aditya Kumar Kasinadhuni wrote: > Hi all, > > Can anyone please help me with answers to a couple of questions I have? > > First, to calculate the total intracranial volume why is it necessary to multiply the outputs from the following command > fslstats filename -V -M > I was thinking the the image "filename_struc_GM.nii.gz" consisted of only gray matter and hence knowing the voxel size and number of voxels would be sufficient to calculate the GM volume. If so, can we not extend this to WM and CSF to calculate their volumes and eventually the TIV?? > > Second, > Is there any place where I can find the description of all the files listed in the /struc directory? I tried to look at the documentation but I think it lists only that from the /stats directory. > > Can someone please throw some light on these doubts that I have? > > Thank you. >