Hi Israel,

Just to add to that, and as I know you use the Diamond synchrotron, the device mentioned is available (on advance request) on the beamline I02.


-- David

On 1 May 2011 19:50, Martin Weik <[log in to unmask]> wrote:
Dear Israel,

there is also a dedicated humidity device developed at EMBL/ESRF Grenoble that allows for systematic dehydration experiments to be carried out at room temperature and soon at 4°C. Here is the primary reference:

Sanchez-Weatherby, J., Bowler, M.W., Huet, J., Gobbo, A., Felisaz, F., Lavault, B., Moya, R., Kadlec, J., Ravelli, R.B. and Cipriani, F. (2009) Improving diffraction by humidity control: a novel device compatible with X-ray beamlines. Acta Crystallogr D Biol Crystallogr., 65, 1237-1246.



Le 01/05/11 19:32, Israel Sanchez a écrit :

Hi folks,

I am currently impressed by the efficiency of dehydration treatments over the diffraction capacity of our crystals in one particular condition. Without any treatment the crystals seldom diffract to 20-30A but in our last synchrotron trip the very same crystals, after been incubated with increasing concentration of low molecular weight PEGs diffracted to 6A.

I was wondering if anyone has studied these effects in a systematic way. Does anyone on the ccp4bb knows  references or has any experience/pseudo-religious believes that do not care to share with the community about this particular topic?

Thank you very much in advance!!!!

 Israel Sanchez Fernandez PhD
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 0QH, UK