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Hi Cory,
I am afraid to say that Pichia and Saccharomyces are tough cells to break
compared to bacteria. We also purifiy membrane proteins in yeast in my lab
so we have gone through this process.
With regular yeast we can break in an emulsiflex but it takes some hard work
and this is not really viable option on a large scale once you process
several liters of cell culture.
So we use a bead-beater ( from BIOSPEC) and glass beads (0.5 mm for yeast,
the diameter depends on the kind of organism you want to disrupt). it works
well and is quite fast , however you have to deal with the bead cleaning
part and the losses due to the mass of liquid trapped in the beads (rinsing
of beads with buffer is fine but it results in an increase burden at the
membrane centrifugation step)
It takes some optimization (mass of beads/mass of cells processed), but once
this is is set this is probably the way to go,
Bead beaters come in various sizes depending on the volumes you want to
process.
It is just a blender after all.

Hope this helps.

Best regards

-- 
Pascal F. Egea, PhD
Assistant Professor
UCLA, David Geffen School of Medicine
Department of Biological Chemistry
314 Biomedical Sciences Research Building
office (310)-983-3515
lab      (310)-983-3516
email     [log in to unmask]