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Hi All (most probably dan or wayne) I think this may be a bug when i run peak separator it finds the peaks but there is an obvious systematic offset from the true peak position as shown in the attached figure regards gary -- ------------------------------------------------------------------- Dr Gary Thompson [Homans Lab Research Coordinator] Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, West-Yorkshire, UK Tel. +44-113-3433024 email: [log in to unmask] Fax +44-113-3431935 -------------------------------------------------------------------