From: Roger Ekins <[log in to unmask]>
To: [log in to unmask]
Sent: Wed, 30 June, 2010 16:37:31
Subject: Re: Sensitivity - yet again!
Re: Sensitivity - yet again!
Dear Mohammad
The authors set out their reasons on page 1 of their paper
(attached)
With best regards
Roger
"Aside from summarizing the
clinical need for ultrasensitive assays..."
Sorry Prof Ekins, I don't have
access to this journal, can you please let us know what are the
clinical needs/justification to measure any of the current analytes in
subfemtolitre concentrations. Honestly, this is a genuine query and I
don't want to distract any body from the debate around
the definition of method sensitivity.
Best regards
Mohammad
Dr. M A Al-Jubouri, MB ChB, MSc, FRCP
Edin, FRCPath
Consultant Chemical Pathologist
From: Roger Ekins <[log in to unmask]>
To: [log in to unmask]
Sent: Wed, 30 June, 2010 11:01:50
Subject: Sensitivity - yet again!
Anyone - including our friends at IUPAC - doubting that the term
"sensitive" (as applied to a method of measurement,
including an assay) is widely recognized throughout science
(including the most prestigious journals) as implying that the method
possesses a low detection limit should perhaps peruse a recent paper
in Nature Biotechnology (Rissin et al. Single-molecule enzyme -linked
immunosorbent assay detects serum proteins at subfemtomolar
concentrations. Nature Biotech. 28. 595 - 599 (June) 2010.)
Aside from summarizing the clinical need for ultrasensitive
assays, this paper indicates the future course of development in the
field of clinical chemistry, albeit it conspicuously fails to mention
that methods of greater sensitivity (and simplicity) have been
developed more than 18 years by my colleagues and myself in
association with Boehringer Mannheim GmbH using microarray -based
methods, fluorescent microspheres and confocal microscopy
--
Prof Roger Ekins, PhD DSc FRS
Windeyer Instituteof Medical Science
University College London
London W1T 4JF
Phone +44 20 7679 9410
Fax +44 20 7679 9407
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--
Prof Roger Ekins, PhD DSc FRS
Windeyer Institute of Medical Science
University College London
London W1T 4JF
Phone +44 20 7679 9410
Fax +44 20 7679 9407
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
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