Oh yes and: 3. analysis cares about the sign of alpha and beta peaks in e.g. HNCACB. If you have alpha -ve and beta +ve the quick fix is to reprocess your data with a 180 degree 0 order phase correction and recalculate the peak intensities. -- Dr. Brian O. Smith ---------------------- B Smith at bio gla ac uk Division of Molecular & Cellular Biology, Faculty of Biomedical & Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK. Tel: 0141 330 5167/6459/3089 Fax: 0141 330 4600 ------------------------------------------------------------------- The University of Glasgow, charity number SC004401