Lisa Wang wrote: > hi all, > I have one structure contains dsRNA with two phosphate group at 5'.. I > named > the first residue GDP and got a cif file by running phenix.elbow. Then I > refine my structure by running phenix.refine my.pdb my.cif. But the fist > and > the second residue do not link with each other, that is no bond between > O3* > of the fist residue and P of the second residue. > Please give me some idea. Thanks > You have to explicitly link them. Something like the following: geometry_restraints.edits { bond { action = *add delete change atom_selection_1 = "chain A and resid 1 and name O3\\*" atom_selection_2 = "chain A and resid 2 and name P" symmetry_operation = None distance_ideal = 1.59 sigma = 0.01 } angle { action = *add delete change atom_selection_1 = "chain A and resid 1 and name C3\\*" atom_selection_2 = "chain A and resid 1 and name O3\\*" atom_selection_3 = "chain A and resid 2 and name P" angle_ideal = 120 sigma = 1 } }