If you want to just reduce the volume/concentrate proteins, you can use Millipore’s Stirred cell (high-output stirred cell, 2,000 ml capacity).
From: CCP4 bulletin
board [mailto:[log in to unmask]] On
Behalf Of Xuan Yang
Sent: Thursday, September 03, 2009 5:05 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale
Dear Dr Ku,
It was a wonderful idea! However, the refolding buffer contained 500mM L-Arg and 1mM EDTA.
And I want to try other affinity columns, just don't know what resin would be appropriate.
2009/9/3 Shao-Yang Ku <[log in to unmask]>
Can you put a 6His-tag on your protein and add some Ni-NTA beads to your solution to capture (and concentrate) the properly folded molecules?
Quoting "Xuan Yang" <[log in to unmask]>:
I am working on protein refolding via dialysis in large volumn
(typically 2~4 litters). It was problematic when I wanted to concentrate the
solution to at least less than 500ml. If you know any device appropriate for
such task, please help me out:)
Thanks in advance!