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If you want to just reduce the volume/concentrate proteins, you can use Millipore’s Stirred cell  (high-output stirred cell, 2,000 ml capacity).

 

Zhongren Wu

 

 


From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Xuan Yang
Sent: Thursday, September 03, 2009 5:05 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale

 

Dear Dr Ku,

 

It was a wonderful idea! However, the refolding buffer contained 500mM L-Arg and 1mM EDTA. 

 

And I want to try other affinity columns, just don't know what resin would be appropriate.

 

Sincerely,

 

Xuan Yang


 

2009/9/3 Shao-Yang Ku <[log in to unmask]>

Can you put a 6His-tag on your protein and add some Ni-NTA beads to your solution to capture (and concentrate) the properly folded molecules?



Quoting "Xuan Yang" <[log in to unmask]>:

Dear All,

I am working on protein refolding via dialysis in large volumn
(typically 2~4 litters). It was problematic when I wanted to concentrate the
solution to at least less than 500ml. If you know any device appropriate for
such task, please help me out:)

Thanks in advance!

Sincerely,

Xuan Yang