Hello Joe, I recently converted the result of my matlab computation to .nii using spm_write_vol shown below: Write an image volume to disk, setting scales and offsets as appropriate FORMAT V = spm_write_vol(V,Y) V (input) - a structure containing image volume information (see spm_vol) Y - a one, two or three dimensional matrix containing the image voxels V (output) - data structure after modification for writing. Though my matlab file was a 3D, I dont know if you can use this for 4D, but you can convert from 4D to 3D if need be. FORMAT V = spm_write_vol(V,Y) Y is your Matlab file and V is your header information. Format V is your nifti file, which is your output. You have to choose V from the SPM template. I hope this helps. Regards, Moses Moses Olufemi SOKUNBI, SINAPSE PhD student, Aberdeen Biomedical Imaging Centre, Research MRI, Lilian Sutton Building, Foresterhill, Aberdeen, AB25 2ZD. Scotland, United Kingdom. Office Tel: +44 (0)1224 559731 Mobile: +44 (0)7551 408 357 Email: [log in to unmask]<mailto:[log in to unmask]> ________________________________ From: SPM (Statistical Parametric Mapping) [[log in to unmask]] On Behalf Of Joseph Whittaker [[log in to unmask]] Sent: 15 May 2009 14:33 To: [log in to unmask] Subject: [SPM] converting to nifti Hi Does anyone know how to convert matlab files into .nii files that can be read by SPM. I am trying to use SPM to analyse some optical imaging data. I have arranged my data into a 4D struct [x y z t] although as it is not fMRI data there are only 2 spatial dimentsions so I have 60 frames of 512x512 14 bit images arranged in a matlab file as (512,512,1,60). I used the make_nii function that is available in the MatLab NIFTI and ANALZYE toolbox, and although it seems to work and it creats an image file with a header etc, when I save it as a .nii file I am still unable to open it with SPM. Maybe I'm barking up the wrong tree in thinking I can treat my optical imaging data like fMRI data, but my supervisor is convinced I can use SPM with optical imaging data. Any advice ot information would be much appreciated Thanks Joe The University of Aberdeen is a charity registered in Scotland, No SC013683.