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Dear All

I got recently a comment on a paper review suggesting me to perform CD 
spectroscopy to determine the alpha-helical content of a mutant protein 
involved in a human disease. The diferences between the wild type and 
the mutant protein are just 17 aminoacids. My question is if CD 
spectroscopy could detect secondary structure differences between the 
wild-type and the mutant proteins based on this aminoacid difference. 
Let's say, is it possible to detect by CD the presence or absence of a 
17 aa helix in a protein of 400 aa.

I would appreciate your opinion

All the best

Francisco J. Enguita
ITQB
Oeiras
Portugal