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Dear all,
I was wondering if any of the users of the
above assay for Anti-Müllerian Hormone could help clarify something.
Occasionally we get samples that have
absorbances close to 0 or even -0.001 or so. Now, I just thought that this was
due to the assay not being sensitive enough at these low levels and thought
that such samples had very low AMHs anyway. However, we brought this up with
Beckmann-Coulter and they claim that our blank seems to have very high
absorbance and that’s what renders the assay insensitive at this level. The
kit insert states that the absorbance of the blank is expected to be ~0.03,
while what we get is about 0.07-0.08. We wondered if it was just peculiar to
the lot No we were using (890142) but now we did our 1st batch with
a new lot (990508) and the blank absorbance was 0.066 - pretty much the same.
I’m wondering what absorbances other
users get for the blank and whether you see samples with A close to 0. We run
the assay on the DS2 ELISA system.
Many thanks in anticipation.
Angela
Angela
Kremmyda
Senior Biochemist
Dept of Clinical Biochemistry
SY3 8XQ
Tel: 01743 261158
Fax: 01743 261159