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N-terminal sequencing / MS for intact mass analysis are the only ways that I
can think of.....

Matt

2008/7/1 Klaus Futterer <[log in to unmask]>:

> We have a 150 kDa protein that reproducibly crystallises at one of the
> Hampton Screen conditions. However, we know from SDS gel analysis that the
> crystals contain only a 45 kDa fragment, that forms through proteolytic
> cleavage over time. We would like to determine the sequence boundaries of
> the fragment.
>
> We believe a combination of N-terminal sequencing plus MS analysis might
> give us the information we need, but I was wondering whether the ccp4bb
> community has other suggestions.
>
> Klaus
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> ---------------------------------------------------------------------
>
>                    Klaus Fütterer, Ph.D.
>
> School of Biosciences             P: +44-(0)-121-414 5895
> University of Birmingham          F: +44-(0)-121-414 5925
> Edgbaston                         E: [log in to unmask]
> Birmingham, B15 2TT, UK           W: www.biochemistry.bham.ac.uk/klaus/
> ---------------------------------------------------------------------
>




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Matthew LH Chu
PhD Student
School of Pharmacy and Pharmaceutical Sciences
University of Manchester
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