We have an Hitachi 917 analyser which performs our conjugated bilirubin
assays (Roche H917 reagents), mostly on neonates. Most of the samples have
some degree of hemolysis (as measured by the analyser, not always visible),
and interference studies we have done show that the assay is quite
sensitive to hemolysis, with results often coming out as negative numbers.
Our interference studies have also shown that the degree of interference is
related to both the degree of hemolysis and the true conjugated bilirubin
concentration, and thus far we have not been able to construct any
mathematical way to apply a correction.
I would like to know how other labs get around this problem. I have tried
an acidification of the R1 reagent (as suggested by another lab) but
preliminary results show a lessening of the effect, but not totally
removing it.
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Frank Alvaro
Dept of Clinical Chemistry
South Western Area Pathology Service
Liverpool Hospital
Sydney
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