> Sten Öhman wrote:
> -
> -
> >>>I don't know why, but in my experience the outer positions often differ
> significantly both with respect to blank values and color intensity compared
> with the inner positions.
> ---------------------------------------------------------------------------
> This was certainly a well known effect when I used to do rather more ELISAs
> some 10-15 years ago, and we always used to leave the outer wells unfilled for
> this reason. It was put down to manufacturing difficulties. I thought that
> more recently the quality of plates had improved but obviously not yours. It
> might be worth compalining to the supplier and trying a different source if
> possible.
> Unfortunately ELISA is often used to produce positive/negative results (e.g.
> for immunology) which has always seemed to me to be slightly dubious. The lack
> of a numerical result may tempt users to be less than critical about QC
> issues such as assay reproducibility. In any case, what does a negative result
> for an autoantibody (or anything) mean? Does it mean totally absent, below the
> limit of detection or within the normal range (or something else I haven't
> thought of)? "Positive" could be equally misleading.
If any immunologists are reading this, no offense. Please feel free to
be rude
back about my discipline, biochemistry, if you can.
Steve Frost
>
> [log in to unmask]
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