Hi. I have a case that is similar to the splicesome example in Nakane et
al. (https://elifesciences.org/articles/36861). I would like to follow
up the first multi-body run with signal subtraction of 3 of 4 bodies for
subsequent 3D classification and refinement of the remaining body.
Takanori, will you elaborate on the procedure summarized here
(particularly the relion_flex_analyse details):
"...we then used the relion_flex_analyse program to subtract the other
three bodies from all experimental particle images, and performed a
focused 3D classification on the SF3b without alignments."
Mike
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Michael D. Purdy
University of Virginia
480 Ray C. Hunt Dr., Rm 320
Charlottesville, VA 22903
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