Thanks for your reply, I had another data set from same sample with 20 degree tilt, I got 4.4 A structure in relion, because preferred sample orientation, I need 40 degree tilt data to get better map.
Do you think that I put both data sets together and do bayesian polishing or I need do a couple of ctf refinements before bayesian polishing?
Best wishes,
Haiyan Zha
Department of Biochemistry and Molecular Genetics
Voice: 858-869-3633
On 3/26/19, 3:34 PM, "Takanori Nakane" <[log in to unmask]> wrote:
>Hi,
>
>At 14 A, there is no point running Polishing (yet) and
>you cannot reliably fit B factors.
>
>If you believe the resolution is limited by the defocus gradient,
>run several cycles of CtfRefine and Refine3D (see hemagglutinin example
>in our RELION3 paper) or use tilt-aware CTF estimation programs
>(e.g. Warp, CTFTILT, GoCTF).
>
>Best regards,
>
>Takanori Nakane
>
>On 2019/03/26 21:30, Zhao, Haiyan wrote:
>> Because it is a tilt 40 degree data set on K3 detector with 200KV Arctic, so far the resolution is 14A after 3D refinement
>> Resolution range is default,
>> Dose rate is 1.34 e-/A2,
>>
>>
>> Haiyan Zhao
>> Department of Biochemistry and Molecular Genetics
>> Voice: 858-869-3633
>>
>>
>>
>>
>>
>>
>>
>> On 3/26/19, 3:17 PM, "Takanori Nakane" <[log in to unmask]> wrote:
>>
>>> Hi,
>>>
>>> What is the resolution of your structure?
>>> What is the resolution range you used to fit B factors?
>>> What is the dose rate?
>>>
>>> Best regards,
>>>
>>> Takanori Nakane
>>>
>>> On 2019/03/26 20:41, Zhao, Haiyan wrote:
>>>> I collected a tilt data set, when I run bayesian polishing for all
>>>> particles, I got the b factor like I attached the pdf file, I don’t know
>>>> what happened? Any suggestions. Thanks
>>>>
>>>> Haiyan Zhao
>>>>
>>>> Department of Biochemistry and Molecular Genetics
>>>>
>>>> Voice: 858-869-3633
>>>>
>>>>
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>
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