Hi,
The advantage of non-gain-corrected data is that it is easier to
compress. The quality of motion correction is the same.
Unfortunately, you cannot save non-gain corrected data from Falcon.
It is available only for K2/K3.
Best regards,
Takanori Nakane
On 2018/12/12 2:58, 류범한 wrote:
> Hello, CCPEM.
>
> So far I have always collected gain-corrected data with EPU (& Titan Krios+Falcon3 / FEI).
>
> But in somewhere I read that ‘non-gain-corrected data' would be better in motion tracking with separately prepared gain reference image. So I am about to check how much it would be helpful, but I never tried this before.
>
> Regarding this, I have several questions.
>
>
> 1. What is the actual advantage of ’non-gain-corrected data’? in what terms exactly?
> (Simply tracking algorithms of motion correction softwares are slightly better than EPU?)
>
> 2. In practice, how much is it advantageous? in what terms?
> (Especially in the aspect of final resolution of refined 3D map)
>
> 3. How can I collect non-gain-corrected data ?
> By selecting ‘Bias’ option of Bias/Gain correction setting in CCD/TV camera TAB on TEM user interface?
>
> 4. How can I prepare gain reference separately and correctly?
> - Simply take from Falcon Reference manager? But file extension is .RAW other than .mrc. :(
> - Set the imaging condition exactly same with that of data collection, and just take gain reference image w/o sample or in empty hole area? (then check it via TIA?)
>
>
> Thanks in advance.
>
>
>
> Bum Han Ryu
>
> Postdoctoral Researcher
> Korea Basic Science Institute (KBSI)
> 161 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si
> Chungcheongbuk-do, Republic of Korea
>
> Office) +82-43-240-5329
> Mobile) +82-10-7119-0505
>
>
>
>
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