Hi Sean,
Does this happen in the latest version of RELION 3.0 beta?
> The reason I think it failed is related to the fact that in the
> corrected_micrographs.star file the _rlnAccumMotionEarly column has a
> value of 0.000000 for all entries (= problem making histogram ??).
I fixed this bug on August 14 th (commit ee189e90 and 9d657c5).
If this still happens, please let me know. If possible, please send me
your corrected_micrographs.star off-list.
Best regards,
Takanori Nakane
> This morning I had a motion correction job fail while generating the
> logfile.pdf with a segmentation error. I think it failed creating the
> corrected_micrographs_hist_rlnAccumMotionEarly.eps file as it is blank
> when viewed with gs while the other 3 eps files are fine. The reason I
> think it failed is related to the fact that in the
> corrected_micrographs.star file the _rlnAccumMotionEarly column has a
> value of 0.000000 for all entries (= problem making histogram ??). I am
> guessing this value is 0 because the movies are 19 frames with a dose of
> 2.43 e/A/frame, so when calculating which frames to use for the Early
> Motion it cuts off after the first frame as the ?dose_motionstats_cutoff
> sets by default the dose to distinguish early/late global accumulated
> motion at 4 which in my case is reached in the second frame.
>
> So basically my question is: Is the rlnAccumMotionEarly motion data used
> any where else later in the pipeline such that I should rerun motion
> correction but increase the ?dose_motionstats_cutoff so I can get
> reasonable numbers for _rlnAccumMotionEarly? Is there an optimal number
> of frames to be using ?
>
> Many thanks for your help and also Relion in general,
>
> Sean
>
> PS continuing the motion correction job with ?dose_motionstats_cutoff 10
> completes with no issue.
>
>
>
>
>
> Center of Excellence Severo Ochoa (2017-2021)
>
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