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CCPEM  August 2018

CCPEM August 2018

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Subject:

relion2.1 autopicking test error (warning)

From:

Jing Liu <[log in to unmask]>

Reply-To:

Jing Liu <[log in to unmask]>

Date:

Tue, 28 Aug 2018 01:04:13 +0100

Content-Type:

text/plain

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Parts/Attachments

text/plain (22 lines)

Hi Dear CCPEM community, 

I am learning relion and am trying to go through the tutorial (2.1). I have encounter a problem when I tried auto-picking 2 micrographs I selected from the tutorial dataset.   (I didn't use gpu acceleration and all other parameters are the same as in the tutorial (eg. 1 MPI processor). ) I saw the same warning on a github post (https://github.com/3dem/relion/issues/171), but I am not sure whether these are related. Could anyone give me some idea of what went wrong and how could I fix the problem? Thank you very much. Below are the warning I saw, and I could not open the result star file. 
 
+ Using (micrograph) pixel size from input STAR file of 3.54 Angstroms
 + Run autopicking on the following micrographs: 
    * MotionCorr/job005/Micrographs/Falcon_2012_06_12-16_44_07_0.mrc
    * MotionCorr/job005/Micrographs/Falcon_2012_06_12-17_14_17_0.mrc
 + WARNING: Requested rescale of micrographs is 690 pixels. The largest prime factor in FFTs is 173
 + WARNING: Will change rescaling of micrographs to 684 pixels, because the prime factor then becomes 19
 + WARNING: add --skip_optimise_scale to your autopick command to prevent rescaling 
 + WARNING: The calculations will be done at a lower resolution than requested.
 Initialising FFTs for the references and masks ... 
   6/   6 sec ............................................................~~(,_,">
 Autopicking ...
  44/  44 sec ............................................................~~(,_,">

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