Hi Dear CCPEM community,
I am learning relion and am trying to go through the tutorial (2.1). I have encounter a problem when I tried auto-picking 2 micrographs I selected from the tutorial dataset. (I didn't use gpu acceleration and all other parameters are the same as in the tutorial (eg. 1 MPI processor). ) I saw the same warning on a github post (https://github.com/3dem/relion/issues/171), but I am not sure whether these are related. Could anyone give me some idea of what went wrong and how could I fix the problem? Thank you very much. Below are the warning I saw, and I could not open the result star file.
+ Using (micrograph) pixel size from input STAR file of 3.54 Angstroms
+ Run autopicking on the following micrographs:
* MotionCorr/job005/Micrographs/Falcon_2012_06_12-16_44_07_0.mrc
* MotionCorr/job005/Micrographs/Falcon_2012_06_12-17_14_17_0.mrc
+ WARNING: Requested rescale of micrographs is 690 pixels. The largest prime factor in FFTs is 173
+ WARNING: Will change rescaling of micrographs to 684 pixels, because the prime factor then becomes 19
+ WARNING: add --skip_optimise_scale to your autopick command to prevent rescaling
+ WARNING: The calculations will be done at a lower resolution than requested.
Initialising FFTs for the references and masks ...
6/ 6 sec ............................................................~~(,_,">
Autopicking ...
44/ 44 sec ............................................................~~(,_,">
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