Hi,
Because each subunit moves independently, you have to
run multibody refinement in C1. Since multibody refinement
is continuation from Refine3D, the consensus refinement must
also be carried out in C1.
I know this is limitation in some cases. For example,
each subunit might adapt different orientations, breaking
the symmetry, but they may remain rigid
(i.e. no within-domain deformations). Ideally, the references
for each subset should be common in such cases.
We are working to extend multibody refinement.
Best regards,
Takanori Nakane
On 2018/08/22 19:44, Sara Weaver wrote:
> Is there any documentation about using Relion 3 MultiBody Refinement for a
> particle with symmetry? Would applying symmetry violate the assumptions of
> the algorithm?
>
> I've run multibody refine from a consensus refinement I did in C1 and
> treated the subunits as separate bodies. I clearly observe continuous
> structural heterogeneity, so is applying symmetry to these particles at the
> level of consensus refinement inappropriate?
>
> Cheers,
> Sara Weaver
>
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