>>> 12.07.2018 at 18:01:
> Up until recently I had always seen 2D classes similar to looking at
> different angles of a solid object
just keep in mind that the instrument you are operating is a
transmission electron microscope --- isn't it?
thus, in your TEM images , you generate projections thru the almost transparent 3D volume of your objects. (in case , you are looking at proteins or DNA or any kind of 'light' biomolecules).
it is a projection, nothing else. Does this help?
"I see a surface" - no, --> you see a projection thru the 3D volume of your object, in the original image and also in your 2D classes.
HTH. Reinhard
--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail [log in to unmask]
office: VKL 3.1.29
Next microscopy conferences:
- 19th International Microscopy Congress, Sydney; 9-14 Sept 2018
http://imc19.com/
- Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin
- EMC2020 in Kopenhagen (European conference)
- MC2021 in Vienna (D-A-CH conference)
- next Microbiol. conferences:
VAAM 17.-20.03. 2019 Mainz
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