Hi,
This is a known problem of RELION 2.1 and will be fixed in the next version.
Meanwhile, you can change _rlnSgdSubsetSize in optimiser.star to -1
to disable subsets before continuing.
Best regards,
Takanori Nakane
> Dear Sjors and all,
>
> when continuing a Class3D run (e.g. 10 classes) *with subsets* it starts
> to make subsets again before it continues 'normally'. Is this on purpose
> and if so, what does it use to make the subsets? I noticed that the
> course of the separation into the classes was somewhat unexpected (one
> class became much bigger as expected from the interrupted run and the
> other ones became very small.
>
> Thanks, Dieter
>
> ------------------------------------------------------------------------
> Dieter Blaas,
> Max F. Perutz Laboratories
> Medical University of Vienna,
> Inst. Med. Biochem., Vienna Biocenter (VBC),
> Dr. Bohr Gasse 9/3,
> A-1030 Vienna, Austria,
> Tel: 0043 1 4277 61630,
> Fax: 0043 1 4277 9616,
> e-mail: [log in to unmask]
> ------------------------------------------------------------------------
>
>
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