I've used e2motion.py in the past to get an animation of the movement range of my protein however the last couple of times trying it I cant seem to get it to work;
when using;
e2motion.py --path m2d_01 --iter 1 --threads=16
I get the message:
Warning: no particle alignment data found for iter= 1
I had previously used the:
e2a2d_align.py particles/particleMRC.hdf particles/RefImageMRC.hdf --align rotate_translate_tree --aligncmp ccc --cmp ccc --threads=16
I thought this had worked as it created the directory 'm2d_01' which contained three files:
0_a2d_parms.json
particle_parms_01.json
refimg_01.hdf
Any idea where this might be going wrong?
The particle.hdf was an mrc stack containing 1663 particle images. The Reference Image was also an mrc stack from a single Relion Class Average containing 191 images. Should the Reference image be just a single class-average image and if so do you know how I would get this from relion?
Thanks for your help,
Sonya
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