Hi Dieter,
Some particles were already used in a 3D refinement job earlier, and
hence have been assigned a random subset of 1 or 2. Other particles have
apparently been added in later and do not have a random subset. The
refine program (which uses rlnRandomSubset, the reconstruct doesn't)
complains about this, basically as a protection against not doing what
you would want to do (which is keep random half-sets always the same).
If you know what you are doing, you could just randomly assign 1 or 2 to
the particles that currently have a 0 in that column.
HTH,
Sjors
On 05/28/2018 01:52 PM, Dieter Blaas wrote:
> Dear colleagues,
>
> running 3D auto-refine with Relion-2.1 I get the below error:
>
> ERROR:
> ERROR Experiment::divideParticlesInRandomHalves: some random subset
> values are zero and others are not. They should all be zero, or all
> bigger than zero!
> File: /opt/relion/src/exp_model.cpp line: 207
>
> However, when running the same dataset with relion_reconstruct it runs
> to completion without any error. What might be wrong?
>
> Thanks, Dieter
>
> ------------------------------------------------------------------------
> Dieter Blaas,
> Max F. Perutz Laboratories
> Medical University of Vienna,
> Inst. Med. Biochem., Vienna Biocenter (VBC),
> Dr. Bohr Gasse 9/3,
> A-1030 Vienna, Austria,
> Tel: 0043 1 4277 61630,
> Fax: 0043 1 4277 9616,
> e-mail: [log in to unmask]
> ------------------------------------------------------------------------
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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