Can someone please clarify the meaning of minimum inter-particle distance during autopicking for me? Specifically, if using a particle diamaeter of 150 angstroms in the picking display, I would have thought that specifying a value of 150 angstroms for this parameter would have just allowed particles whose circles in the display are tangent but do not overlap; larger values should prevent neighboring particles from even touching (assuming they're spherical). Sadly, this seems not to be the case. While the number of particles picked decreases with increasing values of minimum inter-particle distance, a setting of 200 or even 250 seems to not be sufficient to prevent tangent or even slightly overlapping particles.
Regards,
-jh-
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