Hi,
Is your structure aligned to the symmetry requirement?
That is, the seven-fold rotation axis must be aligned to the Z axis.
Best regards,
Takanori Nakane
On 2018/03/15 22:16, #ARVIND KUMAR KHANDELIA# wrote:
> Hi everyone
>
>
> I am new to SPA. I have a basic doubt.
>
>
> How can I impose symmetry (D7 in particular) during refinement. I have done 3D classification using C1 symmetry but the resolution is ~ 5A. While the reported resolution for dataset is ~ 3A
>
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> I have tried using D7 in with different regularisation parameter T (T=6 or T=7) in relion 2.0.3 but I am not getting proper reconstruction.
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> In addition is there any difference in imposing symmetry in relion 2.0.3 and relion 2.1
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>
> Thank You
>
> Best regards
> Arvind Kumar
> Nanyang Technological University
>
> <http://www2.sbs.ntu.edu.sg/staff/ggrueber/>
>
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