Hi Michael,
For option 1) you'd need the final _data.star, which you should split
into 2 (e.g. using awk) based on the value in the rlnRandomSubset
column. You can then use relion_reconstruct (with option --ctf and
whatever --sym if present) to reconstruct the 2 half-maps without
performing an additional expectation step. This has been observed to
give slightly lower resolutions though.
HTH,
Sjors
On 07/17/2017 04:33 PM, Michael Woodson wrote:
> Hi Everyone:
> The computer I am using for auto-refine always crashes at the last
> iteration, with an out-of-memory error from /home/builder/relion-2.0.3/
> src/gpu_utils/cuda_backprojector.cu
> I have tried continuing the job with GPU acceleration turned off, but for
> some reason it continues with more iterations of alignment. This is
> extremely slow on this machine without GPU acceleration. I'd like to either:
> 1) generate unfiltered half-maps myself, ie with relion_reconstruct. I
> don't know what file to use for input. I had assumed the
> run_it##_half1_model.star files, but they don't work.
> 2) continue the job on CPUs in such a way that only the final
> reconstruction steps are performed, not alignment.
>
> thanks!
>
> Michael Woodson,
> Staff Scientist
> UTMB Galveston, TX, USA
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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