Dear all,
I'm processing a data set with Relion. Apparently 3D refinement, 3D classification and previous steps went fine, but after 3D refinement the post-processing resulted in a weird map; the shape is recognizable (a ribosome) but, the whole map seems to be affected by a layer pattern (image attached).
I've realized that also the unfil half-maps resulting from the 3D refinement have the same weird pattern, so I think the problem is coming from these maps. Half-maps before the last iteration, as well as the final map of the 3D refinement look ok; only the unfil half-maps show this pattern. Does anybody know what could be going on?
Thanks in advance.
Best,
Miguel.
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