I am trying to to 2D classification on negatively stained samples. I have manually picked particles in 10+ micrographs (so I now have 10+ STAR co-ordinate files) but I want to perform a 2D classifcation on all the particles together (ie so that I have enough particles to get good 2D class templates to use fpr auto-picking). Can I do this or do I need to group the micrographs together before I do the picking?
Thanks
Sonya
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