Dear Fengbin,
You cannot correct CTF on individual particles, it is an intrinsic part of
the averaging process.
I would probably use Xmipp to calculate power spectra for all inidivual
particles. You could then average those without bothering about the CTF.
HTH,
Sjors
> Dear Sjors and Aaron
>
> Thank you for your inputs! Then can both CTF and transformation be applied
> to generate a new image stack?
>
> My original purpose is generate a power spectrum for every image in that
> 2D-classification group, and then average them into a one power spectrum
> to estimate the helical symmetry. Is there a relion command can do this?
> Thanks again for your time :)
>
> Best Regards,
> Fengbin
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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