Hi Chancie,
You may also try to add small amounts of detergents, or collect data in
slightly thicker ice.
HTH,
Sjors
On 01/09/2017 10:17 AM, C. Thangaratnarajah wrote:
> Dear all,
>
> I would like to improve the distribution/orientation of my particles.
> I was wondering what strategies people have used to improve it,
> especially for membrane proteins?
>
> Thanks for your input,
>
> Chancie.
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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